In-Vitro Fertilisation (IVF)

The eggs are taken from the female partner and fertilised with the male partner’s sperms under laboratory conditions. The fertilisation and cleavage and further development take place under laboratory conditions and is regularly monitored.

Patients who will benefit from IVF

Female partner's tubal block

Severe endometriosis

Moderately low sperm count

Failed IUI (Failure of fertilisation subsequently cleavage and further development, failure of cleavage or development or failure of implantation)

Fallopian tubes removed due to ectopic pregnancies and other infections

IVF procedure steps
  1. Ovulation induction and follicle tracking
    • Induction is done by stimulating the ovaries
    • This stimulation is done by administrating hormonal medication such as FSH(Follicle stimulating hormone)
    • The female partner’s hormone levels are checked using blood tests
    • Simultaneously ultrasound scans are performed to monitor follicular development
  2. Oocyte maturation
    • HCG injection is administered for the final maturation of the eggs developing in the follicle
  3. Oocyte Recovery
    • Transvaginal ultrasound aspiration is done to retrieve the egg
    • Egg recovery procedure performed under anesthesia
    • The aspirated follicle fluid is examined and the mature eggs are separated and placed in culture media and incubated
  4. Sperm preparation
    • Preparation will separate the immotile and morphologically abnormal sperms
    • The final wash will have motile sperms with normal morphology
  5. Fertilisation and Embryo development
    • The incubated eggs are added to culture dishes with prepared sperms and then incubated
    • The fertilised eggs develops to embryos in laboratory conditions
    • Embryo development is monitored and then optimally developed cleavage stage (day 3) embryos or blastocyst (day 5) embryos are selected to be transferred and rest is frozen

    • Additionally more advanced techniques to assist further embryo development could be used such as, zona thinning and assisted hatching

  6. Embryo transfer and Serum βhCG test
    • Progesterone supplement is given to make the uterine lining ideal for implantation

    • The selected embryos are transferred to the uterine cavity

    • After 14 days pregnancy is identified by a βhCG test and further confirmed through an ultra sound scan